dcfh-da ros staining solution  (Beyotime)

Journal: Redox Biology

Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

doi: 10.1016/j.redox.2026.104198

Figure Lengend Snippet: Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.

Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

Techniques: Staining, Expressing, Transmission Assay, Electron Microscopy, Microscopy, Control

Journal: Redox Biology

Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

doi: 10.1016/j.redox.2026.104198

Figure Lengend Snippet: Observing whether exogenous lactate supplementation attenuates PG's effect on improving MIRI. A-C: Detect LVEF and LVFS in the mouse using echocardiography; D-E: Serum cTnI and CK-MB expression levels; F-G: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; H–I: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); J: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); K: Quantitative analysis of transmission electron microscope images; L: Establish a mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Serum LDH expression level; N: Establish a mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using HL-1 cells to establish an OGD/R model to observe the effect of PG on lactate levels. Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R; & P < 0.05, && P < 0.01, &&& P < 0.001, VS I/R + PG or OGD/R + PG.

Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

Techniques: Expressing, Staining, Transmission Assay, Electron Microscopy, Microscopy, Control

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A Blue: Nuclei. Purple: Mitochondrial. Red: PINK1/Parkin/p62/LC3B. IF staining was performed to detect PINK1, Parkin, p62, and LC3B protein levels (magnification: 100×, scale bar: 15 μm). B Colocalization analysis of p62, LC3B, and mitochondria was performed using ImageJ software. C Colocalization Pearson correlation coefficient analysis were obtained based on IF images of p62 and LC3B ( n = 6). D Western blot analysis of PINK1 and LC3B expression in renal cortical tissues from different experimental groups. Quantification of gray values for Western blotting results in ( D ) was performed using ImageJ ( n = 3). E Blue: Nuclei. Red: E-cadherin/N-cadherin/Vimentin. IF assay was performed to detect E-cadherin, N-cadherin and Vimentin protein levels (magnification: 100×, scale bar: 15 μm). F Quantitative analysis of fluorescence intensity using ImageJ software ( n = 6). G ELISA quantification of CTGF and PDGF secretion in HK-2 cells ( n = 3). H Western blot analysis of E-cadherin, N-cadherin and Vimentin expression in TGF-β1-induced HK-2 cells. Quantification of gray values for Western blotting results in ( H ) was performed using ImageJ ( n = 3). I Representative flow cytometry plots showing the detection of MMP. J Quantitative analysis of flow cytometry fluorescence intensity of ROS ( n = 3). * p < 0.05 vs. Con group, # p < 0.05 vs. TGF-β1 group. a p < 0.05 vs. HP group, b p < 0.05 vs. AST group.

Article Snippet: The cells were then resuspended in 1× JC-1 (C2003S, Beyotime, Shanghai, China) staining solution or 50 μM ROS staining solution (KeyGen Biotech Co., Ltd., Jiangsu, China), and incubated at 37 °C in the dark for 20 and 30 min followed by two washing steps with HBSS.

Techniques: Staining, Software, Western Blot, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Flow Cytometry